![]() ![]() Because protein concentrations can vary over many orders of magnitude in patient or cell line samples, it is desirable to have accurate measurements of protein concentrations over a wide dynamic range. The protein level across the slide is detected by binding of a highly specific and sensitive primary antibody followed by detection using amplification linked to fluorescence, dye deposition, near infrared or nanoshells. A single array slide can be used to measure hundreds of samples for a protein. This technology has been successfully applied in a number of basic and clinical studies (Amit et al., Aoki et al., Fan et al., Pluder et al., Sahin et al., Tibes et al., Yokoyama, et al., ). Examples including anticoagulant and bacterial culture fluids. The initial (parent) sample size (either larger or smaller than the normal) could cause under dilution or over dilution respectively. ![]() If a sample with a particular size is being placed into a fixed amount of diluent. 1 INTRODUCTION The reverse phase protein array (RPPA) is an emerging high-throughput technique in proteomics (for reviews, see Borrebaeck and Wingren, Charboneau et al., Lv and Liu, Poetz et al., Sheehan et al., ). The dilution error is initially related to sample size. Availability: A computer program in R for using serial dilution curve for RPPA data analysis is freely available at. We showed how the method can be used to examine data quality and to obtain robust quantification of protein concentrations. ![]()
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